PAK-CAS ALFALFA: HIGH SEED YIELDING ALFALFA VARIETY OFFERING UNPARALLELED NUTRITIONAL VALUE AND BIOTIC TOLERANCE

Authors

  • Qamar Shakil Principle Scientist, Fodder Research Substation, Ayub Agricultural Research Institute, Faisalabad. Author
  • Amna Kanwal Scientific Officer, Fodder Research Substation, Ayub Agricultural Research Institute, Faisalabad. Author
  • Zohab Asif Scientific Officer, Fodder Research Substation, Ayub Agricultural Research Institute, Faisalabad. Author
  • Muhammad Humaiyon Research Associate, Fodder Research Substation, Ayub Agricultural Research Institute, Faisalabad. Author
  • Zulfiqar Ali Vice Chancellor, University of Agriculture, Faisalabad. Author
  • Atif Farhat Associate Professor, Department of Food Science and Technology, MNS, University of Agriculture, Multan. Author
  • Shamsa Kanwal Scientific Officer, Agricultural Biotechnology Research Institute, Ayub Agricultural Research Institute, Faisalabad. Author
  • Shakra Jamil Principal Scientist, Agricultural Biotechnology Research Institute, Ayub Agricultural Research Institute, Faisalabad. Author
  • Asma Parveen Research Associate, Fodder Research Substation, Ayub Agricultural Research Institute, Faisalabad. Author

DOI:

https://doi.org/10.58475/2025.63.4.1165

Keywords:

Medicago sativa, seed production, high yield, protein content, biotic resistance, DNA fingerprinting, Pakistan

Abstract

Alfalfa seed production is a major challenge all over the globe and the research to combat this phenomenon of low seed yield is limited over the course of time. Its high seed yield, commercial distribution and delivery to farmers at a competitive price are critical issues in Pakistan. Seed yield is governed by complex interaction among genetic potential of a variety and its environments, pollinators, and crop management. Reduce biomass and nutritional value is another limiting factor in impacting the productivity and growth of livestock sector. Under this scenario, scientist of Fodder Research Substation has initiated an effort during the studied year 2013-2015 to develop a new variety of alfalfa (PAK CAS-Alfalfa) by mass selection. Diverse group of seven elite lines (No. 6316, No. 6450 and No. 6455) from local and foreign origin (No. 6433, NO. 34187 No. 9011 and No. 8004) were used to develop promising line FA-01-2019, which was later named as PAK CAS-Alfalfa (2024). This magical variety has proven as high forage yielder (9% higher yield in National Trials) along with high crude protein (22.4%) contents than existing alfalfa varieties. This variety is resistant to Rhizoctonia solani (moderately resistant), alfalfa weevil (3.7% and 3.4%), and stink bug (7.3% and 7.5%) which has deleterious impact on alfalfa fodder and seed yield. This variety (high-quality, nutritive fodder and strong adaptability) has the potential to overcome the constraints in alfalfa production and other fodder related quality parameters.

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References

Abd El-Naby, Z., M. F. M.Sultan, H.S. Abd-El Maksoud and T. K. Abd El Aziz. 2016. Fertility and seed setting of ten alfalfa genotypes. American-Eurasian Journal of Agricultural and Environmental Science. 16 (3): 432-441.

Akber, M.A. and X. Fang. 2024. Research progress on diseases caused by the soil-borne fungal pathogen Rhizoctonia solani in alfalfa. Agronomy. 14 (7): 1-25.

Akmal, M., U. Farid, M. Asim and R. Farhatullah. 2011. Crop growth in early spring and radiation use efciency in alfalfa. Pakistan journal of Botany 43(1):635–641.

Blume, L., S. Hoischen-Taubner and A. Sundrum. 2021. Alfalfa-a regional protein source for all farm animals. Journal of sustainable organic agricultural system, 71(1):1-13.

Faridullah, F., S. Hafeez, T. Ahmed, S. Alam, A. Naz, W. Shah and S. Khalid. 2018. Characterization of Phosphorus in Fresh and Composted Manures of Different Livestock. Polish Journal of Environmental Studies. 27(2):

Feng, Y.P. Y. Shi, M.Y. Zhao, H.H. Shen, L.C. Xu, Y.K. Luo, Y.Z. Liu, A.J. Xing, J. Kang and H.C. Jing.2022. Yield and quality properties of alfalfa (Medicago sativa L.) and their influencing factors in China. European journal of agronomy 141: 126637.

Fernandez, A. L., C.C. Sheaffer, N.E. Tautges, D.H. Putnam and M.C. Hunter. 2019. Alfalfa, wildlife, and the environment. National Alfalfa and Forage Alliance. 632, 633.

Galedar, M.N., A. Jafari, S.S. Mohtasebi, A. Tabatabaeefar, A. Sharifi, M.J. Odogherty, S. Rafiee and G. Richard. 2008. Effects of moisture content and level in the crop on the engineering properties of alfalfa stems. Biosystems engineering, 101(2): 99-208.

Hall, M.H., N.S. Hebrock, P.E. Pierson, J.L. Caddel, V.N. Owen, R.M. Sulc,D.J. Undersander and R.E Whitesides. 2010. The effects of glyphosate tolerant technology on reduced alfalfa seeding rates. Journal of agronomy. 102: 911.

Hall, M.H., C.J. Nelson, J.H. Coutts and R.C Stout. 2004. Effect of seeding rate on alfalfa stand longevity. Journal of agronomy, 96(3): 717-722.

Herreid, J.S., A.R. Tatyana, M.C. Darren, F.B Cockrell, B.P. Frank, and R. Jabbour. 2024. On-farm harvest timing effects on alfalfa weevil across the Intermountain West region of the United States. Frontiers in Insect Science 4: 1324044

Kakaei, M., L. Mazahery and S. Salavati. 2025. Evaluation of Hamedani alfalfa (Medicago sativa L.) seed yield response to planting row spacing and harvesting time of the second cutting fodder. Plant Productions. 48(2): 265-279.

Kaplan, D., R.A. Bien, M. Embaby, A. Skidmore, W. Patterson, E.B. Perry and J. Nair. 2023. Effects of Inoculation of Alfalfa Hay with Pediococcus Pentosaceus and α-Amylase on Hay Temperature, Microbial Ecology, Nutrient Composition, and Feed Value. Journal of Animal Science.101:385

Karar, H., M. Bashir, A. Khaliq, A. Ali, M.J. Alajmi, R.A. and D.M. Metwally. 2021. Stink bug Agonoscelis spp.(Heteroptera:Pentatomidae) An emerging threat for seed production in alfalfa crop (Medicago sativa L.) and their successful management. Saudi Journal of Biological Sciences. 28(6):3477-3482.

Keivani, M., S.S. Ramezanpour, H. Soltanloo, R. Choukan, M. Naghavi and M. Ranjbar. 2010. Genetic diversity assessment of alfalfa (Medicago sativa L.) populations using AFLP journal of crop markers. Australian science. 491-497

Koeritz .J., E. Watkins and N.J. Ehlke. 2015. 4: Seeding rate, row spacing, and nitrogen rate effects on perennial ryegrass seed production. Crop Science. 55 (5):2319.

Latif, A., Y. Sun and A. Noman. 2023. Herbaceous Alfalfa plant as a multipurpose crop and predominant forage specie in Pakistan. Frontiers in Sustainable Food Systems. 7:1126151.

Liao, Z., K. Su, X. Jiang, J. Wang, Y. You, L. Wang, S. Chang, C. Wei, Y. Zhang, C. Li. 2023. Spatiotemporal variation and coupling of grazing intensity and ecosystem based on four quadrant model on the Inner Mongolia. Ecological Indicators. 1 (152):110379

Motarjemi, Y. 2013. Encyclopedia of food Safety. Academic Press.

Missaui, A. M. and J. Young. 2016. Genetic gain from selection and potential for improving alfalfa phosphorus uptake and removal from soils heavily amended with poultry litter. Euphytica 209: 495–506

Putnam, D.H. 2021. Factors Influencing Yield and Quality in Alfalfa. In: Yu, LX., Kole, C. (eds) The Alfalfa Genome . Compendium of Plant Genomes. Springer, Cham. https:// doi.org/10.1007/978-3-030-74466-3_2

Ren, H., S. Ning, A.Yan, Y. Zhao, N. Li and T. Huo. 2025. Response of Alfalfa Yield to Rates and Ratios of N, P, and K Fertilizer in Arid and Semi-Arid Regions of China Based on Meta-Analysis. Agronomy. 15(5): 1093.

Wan, W., Y. Li and H. Li. 2022. Yield and quality of alfalfa (Medicago sativa L.) in response to fertilizer application in China: A meta-analysis. Frontiers in Plant Science. 13: 1051725.

Wang, Y., J. Xie, Z. Sun, Q. Wang and L. Yu. 2024. Effects of Sowing Dates and Phosphorus Application on Seed Yield and Yield Components of Alfalfa. Legume Research: An International Journal, 47(9):1529.

Ye, S., J.X. Zhang, W. Hu, D. Jonathan, G.Z. Zhang, S. Mukamel and J. Jiang. 2020. A machine learning protocol for predicting protein infrared spectra. Journal of American chemical society. 142: 19071–19077.

Zhang, W., N. Grimi, M.Y. Jaffrin, L. Ding and B. Tang. 2017. A short review on the research progress in alfalfa leaf protein separation technology. Journal of Chemical Technology & Biotechnology. 92(12): 2894–2900.

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Published

2026-01-06

How to Cite

PAK-CAS ALFALFA: HIGH SEED YIELDING ALFALFA VARIETY OFFERING UNPARALLELED NUTRITIONAL VALUE AND BIOTIC TOLERANCE. (2026). Journal of Agricultural Research (JAR) ., 63(4), 281-291. https://doi.org/10.58475/2025.63.4.1165